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Exploiting Biomimetic and Luminescence Properties of Multivalent Dendrimer-Semiconductor Nanohybrid Material in Ulta-Low Level Determination of Folic Acid

Abstract

In view of enhanced generation of folate receptors in cancerous cells and diseases linked to deficiency of folic acid, such as anemia, mental devolution, congenital malformation, etc., development of simple method for ultra-sensitive determination of folic acid remains a long-standing issue for practical applications of medicine and biotechnology. Thus, the proposed luminescence based strategy involving multifunctional poly (amidoamine) (PAMAM) dendrimer encapsulated quantum dot (QDs) as probe provides a simple, fast and efficient method of selective determination of folic acid at nano-molar level. Absorption and fourier transform infra-red (FTIR) spectroscopy provide evidence of binding of folic acid with dendrimer amine groups. Emission quenching of dendrimer encapsulated CdS QDs follows a linear Stern-Volmer plot with exceedingly high value of Stern-Volmer constant (KSV = 8.4×106 M-1) facilitating higher detection efficiency. Similar quenching analysis with dendrimer-ZnS QDs showed slightly lower Stern-Volmer constant (KSV = 2.29×106 M-1). The lower probing efficiency of protein or amino acid capping of QDs has been explained through zeta potential measurements. Solvent polarity dependence suggest charge transfer process responsible for emission quenching of CdS QDs, which is static in nature as revealed by lifetime measurement. Determination of folic acid at this low level is not affected by possible interfering molecules, such as vitamic C, vitamin B12 and uric acid. Calorimetric measurements showed that exothermic binding of folic acid with dendrimer follow enthalpy-entropy compensation.The detailed mechanistic aspect of interactions of folic acid with the QD probe helps in better understanding of the detection process, which in turn, can assist in developing a dendrimer based material for image analysis and drug delivery in folate receptor rich cells.

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Publication details

The article was received on 13 Oct 2016, accepted on 05 May 2017 and first published on 12 May 2017


Article type: Paper
DOI: 10.1039/C6AN02284G
Citation: Analyst, 2017, Accepted Manuscript
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    Exploiting Biomimetic and Luminescence Properties of Multivalent Dendrimer-Semiconductor Nanohybrid Material in Ulta-Low Level Determination of Folic Acid

    S. Kundu, S. Maiti, T. K. Das, D. Ghosh, C. N. Roy and A. Saha, Analyst, 2017, Accepted Manuscript , DOI: 10.1039/C6AN02284G

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