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A protocol for rapid, label-free histochemical imaging of fibrotic liver

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Abstract

Mid-infrared microscopy is a non-destructive, quantitative and label-free spectroscopic imaging technique that, as a result of recent instrument advancements, is now at the point of enabling high-throughput automated biochemical screening of whole histology samples. Currently the mid-infrared field is undergoing a paradigm shift that has not been seen since the introduction of scanning Fourier Transform interferometric spectrometers. The latest mid-infrared microscopes are powered by tunable quantum cascade laser (QCL) sources which offer a number of advantages including measurement protocol flexibility, ease-of-use and a greatly enhanced data acquisition speed at high spectral and spatial resolution. In this study we use a wide-field QCL infrared microscope to develop and validate a fast four-frequency protocol for imaging fibrosis in unstained liver tissue. We compare our results to the gold standard Masson's trichrome histochemical staining protocol.

Graphical abstract: A protocol for rapid, label-free histochemical imaging of fibrotic liver

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Publication details

The article was received on 16 Sep 2016, accepted on 15 Nov 2016, published on 15 Nov 2016 and first published online on 15 Nov 2016


Article type: Communication
DOI: 10.1039/C6AN02080A
Citation: Analyst, 2017, Advance Article
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    A protocol for rapid, label-free histochemical imaging of fibrotic liver

    B. Bird and J. Rowlette, Analyst, 2017, Advance Article , DOI: 10.1039/C6AN02080A

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