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Issue 8, 2017
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Digital de-waxing on FTIR images

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This paper presents a procedure that digitally neutralizes the contribution of paraffin to FTIR hyperspectral images. A brief mathematical derivation of the procedure is demonstrated and applied on one normal human colon sample to exemplify the de-waxing procedure. The proposed method includes construction of a paraffin model based on PCA, EMSC normalization and application of two techniques for spectral quality control. We discuss every step in which the researcher needs to take a subjective decision during the de-waxing procedure, and we explain how to make an adequate choice of parameters involved. Application of this procedure to 71 hyperspectral images collected from 55 human colon biopsies (20 normal, 17 ulcerative colitis, and 18 adenocarcinoma) showed that paraffin was appropriately neutralized, which made the de-waxed images adequate for analysis by pattern-recognition techniques such as k-means clustering or PCA-LDA.

Graphical abstract: Digital de-waxing on FTIR images

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Publication details

The article was received on 03 Sep 2016, accepted on 07 Dec 2016 and first published on 07 Dec 2016

Article type: Paper
DOI: 10.1039/C6AN01975G
Citation: Analyst, 2017,142, 1358-1370
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    Digital de-waxing on FTIR images

    F. A. de Lima, C. Gobinet, G. Sockalingum, S. B. Garcia, M. Manfait, V. Untereiner, O. Piot and L. Bachmann, Analyst, 2017, 142, 1358
    DOI: 10.1039/C6AN01975G

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