Jump to main content
Jump to site search

Issue 1, 2017
Previous Article Next Article

Lipid droplets alleviate cadmium induced cytotoxicity in Saccharomyces cerevisiae

Author affiliations

Abstract

Cadmium (Cd) induces oxidative stress that generates reactive oxygen species (ROS) and increased lipid accumulation. However, very little is known about the role of oxidative stress in triacylglycerol (TAG) accumulation. TAG accumulation is deleterious to health and may result in obesity-associated metabolic syndrome. Hence TAG accumulation plays an important role in Cd induced cytotoxicity. The exposure of Wild-type (WT) cells to Cd, resulted in TAG accumulation and also enhanced viability when compared to TAG mutants (dga1Δ, lro1Δ and are2Δ). The inhibition of lipolysis also increased the tolerance of the cells to Cd. Fluorescence microscopy observations using acridine orange and DHR123 staining demonstrated that the TAG deficient mutants showed enhanced cell death and ROS production. The over expression of DGA1 and LRO1 rescued the Cd induced cytotoxicity by enhancing the formation of LDs. Results of this study revealed the possible metabolic link between LDs and oxidative stress in S. cerevisiae.

Graphical abstract: Lipid droplets alleviate cadmium induced cytotoxicity in Saccharomyces cerevisiae

Back to tab navigation

Publication details

The article was received on 04 May 2016, accepted on 03 Oct 2016 and first published on 04 Oct 2016


Article type: Paper
DOI: 10.1039/C6TX00187D
Citation: Toxicol. Res., 2017,6, 30-41
  •   Request permissions

    Lipid droplets alleviate cadmium induced cytotoxicity in Saccharomyces cerevisiae

    S. Rajakumar and V. Nachiappan, Toxicol. Res., 2017, 6, 30
    DOI: 10.1039/C6TX00187D

Search articles by author

Spotlight

Advertisements