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Issue 1, 2016
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miR-122 plays an important role in ochratoxin A-induced hepatocyte apoptosis in vitro and in vivo

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Abstract

OTA can induce hepatotoxicity. Our previous research has shown that miRNAs play important roles in the OTA-induced hepatotoxicity. And miR-122 is the most abundant miRNA in the liver and is involved in diverse biological processes. This study was performed to clarify the role of miR-122 in OTA-induced hepatotoxicity. The expression levels of miR-122 and the target genes were quantified by real-time PCR. The OTA-induced apoptosis of hepatocyte and HepG2 cells was evaluated using a TUNEL kit, a CCK-8 kit, a flow cytometer and Hoechst 33342. miR-122 was inhibited in HepG2 cells. The results revealed that OTA affected rat hepatocyte apoptosis. miR-122 decreased at 4 weeks but increased at 13 weeks in the OTA-treated livers, and increased in the OTA-treated HepG2 cells; and the mRNA levels of CCNG1 and Bcl-w increased at 4 weeks and decreased at 13 weeks in the high-dose OTA-treatment groups and decreased in HepG2 cells. The apoptosis of HepG2 cells displayed a dose-related increase with OTA. However, the inhibition of miR-122 greatly reduced OTA-induced apoptosis. p53 decreased in vivo and in vitro. miR-122 is a primary effector of OTA-induced hepatocyte apoptosis through the CCNG1/p53 pathway and Bcl-w/caspase-3 pathway in vivo and in vitro. And miR-122 plays an important role in OTA-induced hepatotoxicity.

Graphical abstract: miR-122 plays an important role in ochratoxin A-induced hepatocyte apoptosis in vitro and in vivo

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Publication details

The article was received on 08 Apr 2015, accepted on 05 Oct 2015 and first published on 08 Oct 2015


Article type: Paper
DOI: 10.1039/C5TX00104H
Citation: Toxicol. Res., 2016,5, 160-167
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    miR-122 plays an important role in ochratoxin A-induced hepatocyte apoptosis in vitro and in vivo

    L. Zhu, T. Yu, X. Qi, B. Yang, L. Shi, H. Luo, X. He, K. Huang and W. Xu, Toxicol. Res., 2016, 5, 160
    DOI: 10.1039/C5TX00104H

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