Jump to main content
Jump to site search

Issue 42, 2016
Previous Article Next Article

An octamolybdate-metal organic framework hybrid for the efficient adsorption of histidine-rich proteins

Author affiliations

Abstract

The incorporation of a polyoxometalate, octamolybdate (TBA4Mo8O26), into a metal–organic framework, mesoporous chromium terephthalate MIL-101(Cr), produces a novel hybrid Mo8O26@MIL-101(Cr). The covalent interactions of the Mo8O26 moiety in the hybrid with the N-terminal site and the multi-metal binding site of proteins offer Mo8O26@MIL-101(Cr) favorable adsorption performance towards histidine-rich proteins, giving rise to adsorption capacities of 785.6 mg g−1 for HSA and 420.1 mg g−1 for IgG at pH 5.0. Thus, a protocol for the depletion of high abundance proteins from human plasma is proposed. The percentages of serum albumin and IgG are reduced from 50.15% and 13.18% to 7.41% and 3.42%, respectively. Meanwhile, low abundance proteins, e.g., Ig heavy chains, rheumatoid factors, protein IGKV3-11, anti-H1N1 influenza HA, and cDNA FLJ53691, are enriched to a certain extent by ultra-filtration. After the depletion of high abundance proteins, 335 protein species are identified in human plasma, with respect to 265 identified from the raw plasma. This illustrates well the potential of Mo8O26@MIL-101(Cr) in the application of proteomic studies.

Graphical abstract: An octamolybdate-metal organic framework hybrid for the efficient adsorption of histidine-rich proteins

Back to tab navigation

Supplementary files

Publication details

The article was received on 17 Aug 2016, accepted on 23 Sep 2016 and first published on 23 Sep 2016


Article type: Paper
DOI: 10.1039/C6TB02090A
Citation: J. Mater. Chem. B, 2016,4, 6812-6819
  •   Request permissions

    An octamolybdate-metal organic framework hybrid for the efficient adsorption of histidine-rich proteins

    Q. Chen, M. Wang, X. Hu, X. Chen and J. Wang, J. Mater. Chem. B, 2016, 4, 6812
    DOI: 10.1039/C6TB02090A

Search articles by author

Spotlight

Advertisements