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Issue 43, 2016
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An injectable miRNA-activated matrix for effective bone regeneration in vivo

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The delivery of miRNAs that can promote osteogenic differentiation may be promising for bone regeneration. However, low transfection efficiency into cells, especially stem cells, limits in vivo applications. Thus, an injectable miRNA in situ delivery system was designed. miRNAs were encapsulated within nanocapsules which were further entrapped into an O-carboxymethyl chitosan (CMCS) network via electrostatic interactions. In this miRNA-activated matrix system, miRNAs were encapsulated within the polymer shells rather than physically adsorbed onto the surface to avoid leakage of miRNA and offer better stability for cell transfection. microRNA-21 (miR-21) is an osteogenic-related miRNA and it is chosen as the gene model in the current study. Delivery of miR-21 nanocapsules by CMCS significantly promoted the osteogenic differentiation of human umbilical cord mesenchymal stem cells (hUMSCs) as evidenced by up-regulation of osteogenic markers such as alkaline phosphatase (ALP) and runt-related transcription factor 2 (RUNX-2). Also the delivery system promoted bone formation (2.4-fold, p < 0.05) compared to controls. Thus, the current strategy provides not only substantial miRNA protection and better cell transfection efficacy but also has a biodegradable microenvironment with local and sustained gene release, which significantly enhances bone regeneration in vivo.

Graphical abstract: An injectable miRNA-activated matrix for effective bone regeneration in vivo

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Publication details

The article was received on 16 Jul 2016, accepted on 30 Sep 2016 and first published on 03 Oct 2016

Article type: Paper
DOI: 10.1039/C6TB01790H
Citation: J. Mater. Chem. B, 2016,4, 6942-6954
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    An injectable miRNA-activated matrix for effective bone regeneration in vivo

    Y. Meng, C. Liu, J. Zhao, X. Li, Z. Li, J. Wang, R. Wang, Y. Liu, X. Yuan, Z. Cui and X. Yang, J. Mater. Chem. B, 2016, 4, 6942
    DOI: 10.1039/C6TB01790H

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