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Issue 42, 2016
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Fabrication of hydroxyapatite/chitosan composite hydrogels loaded with exosomes derived from miR-126-3p overexpressed synovial mesenchymal stem cells for diabetic chronic wound healing

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Abstract

The exploration of an effective diabetic chronic wound healing process still remains a great challenge. Herein, we used gene overexpression technology to obtain synovial mesenchymal stem cells (SMSCs) and the miR-126-3p highly expressed SMSCs (SMSCs-126). The exosomes derived from miR-126-3p overexpressed SMSCs (SMSCs-126–Exos) with a particle size of 85 nm were encapsulated in hydroxyapatite/chitosan (HAP–CS) composite hydrogels (HAP–CS–SMSCs-126–Exos) as wound dressings. The SMSCs-126–Exos, CS and low-crystallinity HAP nanorods with a length of 200 nm and a diameter of 50 nm are uniformly dispersed within the whole composite hydrogel. The HAP–CS–SMSCs-126–Exos possess the controlled release property of SMSCs-126–Exos for at least 6 days. The released SMSCs-126–Exos nanoparticles stimulate the proliferation and migration of human dermal fibroblasts and human dermal microvascular endothelial cells (HMEC-1). At the same time, the migration and capillary-network formation of HMEC-1 are promoted through the activation of MAPK/ERK and PI3K/AKT. In vivo tests demonstrate that the HAP–CS–SMSCs-126–Exos successfully promote wound surface re-epithelialization, accelerate angiogenesis, and expedite collagen maturity due to the presence of HAP, CS and SMSCs-126–Exos. Therefore, the HAP–CS–SMSCs-126–Exos possess great potential application for diabetic chronic wound healing, and especially provide the possibility of using exosomes derived from modified cells as a new approach to bring wonderful functionality and controllability in future chronic wound therapy.

Graphical abstract: Fabrication of hydroxyapatite/chitosan composite hydrogels loaded with exosomes derived from miR-126-3p overexpressed synovial mesenchymal stem cells for diabetic chronic wound healing

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Publication details

The article was received on 24 Jun 2016, accepted on 23 Sep 2016 and first published on 23 Sep 2016


Article type: Paper
DOI: 10.1039/C6TB01560C
Citation: J. Mater. Chem. B, 2016,4, 6830-6841
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    Fabrication of hydroxyapatite/chitosan composite hydrogels loaded with exosomes derived from miR-126-3p overexpressed synovial mesenchymal stem cells for diabetic chronic wound healing

    M. Li, Q. Ke, S. Tao, S. Guo, B. Rui and Y. Guo, J. Mater. Chem. B, 2016, 4, 6830
    DOI: 10.1039/C6TB01560C

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