Issue 21, 2016

Preparation of graphene nano-sheet bonded PDA/MOF microcapsules with immobilized glucose oxidase as a mimetic multi-enzyme system for electrochemical sensing of glucose

Abstract

A novel amperometric biosensor for glucose detection was fabricated based on a mimetic multi-enzyme system by combing mimetic enzymes (metal–organic frameworks, MOFs) and glucose oxidase (GOx). The microcapsule constructed with polydopamine (PDA) and ZIF-8 using CaCO3 templates could be utilized not only to provide the biocompatible and non-toxic microenvironment to immobilize GOx, but also as an efficient mimetic enzyme because of the peroxidase-like properties of ZIF-8. The graphene nano-sheets (rGO) were bonded to the PDA/ZIF-8 microcapsules to accelerate the electron transfer between the microcapsule and the electrode, and the resulted PDA/ZIF-8@rGO showed excellent electro-catalytic activity towards H2O2. The mimetic multi-enzyme system was fabricated when GOx was immobilized in the as-prepared microcapsule, and the developed GOx/PDA/ZIF-8@rGO/GCE biosensor exhibited extraordinary electro-detection performance for glucose with two wide linear ranges from 1 μM to 1.2 mM and 1.2 mM to 3.6 mM, and the limit of detection was 0.333 μM (S/N = 3). Moreover, the good selectivity for glucose and satisfactory detection results in real samples of the prepared biosensor were also demonstrated.

Graphical abstract: Preparation of graphene nano-sheet bonded PDA/MOF microcapsules with immobilized glucose oxidase as a mimetic multi-enzyme system for electrochemical sensing of glucose

Article information

Article type
Paper
Submitted
31 Jan 2016
Accepted
15 Apr 2016
First published
18 Apr 2016

J. Mater. Chem. B, 2016,4, 3695-3702

Preparation of graphene nano-sheet bonded PDA/MOF microcapsules with immobilized glucose oxidase as a mimetic multi-enzyme system for electrochemical sensing of glucose

Y. Wang, C. Hou, Y. Zhang, F. He, M. Liu and X. Li, J. Mater. Chem. B, 2016, 4, 3695 DOI: 10.1039/C6TB00276E

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