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Issue 2, 2017
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Reversible ratiometric detection of highly reactive hydropersulfides using a FRET-based dual emission fluorescent probe

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Abstract

Hydropersulfide (R–SSH) is an important class of reactive sulfur species (RSS) involved in a variety of physiological processes in mammals. A fluorescent probe capable of real-time detection of hydropersulfide levels in living cells would be a versatile tool to elucidate its roles in cell signalling and redox homeostasis. In this paper, we report a ratiometric fluorescent probe for hydropersulfide sensing, based on a fluorescence resonance energy transfer (FRET) mechanism. This sensing mechanism involves a nucleophilic reaction of a hydropersulfide with the pyronine-unit of the probe, which modulates the intramolecular FRET efficiency to induce a dual-emission change. The reversible nature of this reaction allows us to detect increases and decreases of hydropersulfide levels in a real-time manner. The probe fluorometrically sensed highly reactive hydropersulfides, such as H2S2 and Cys-SSH, while the fluorescence response to biologically abundant cysteine and glutathione was negligible. Taking advantage of the reversible and selective sensing properties, this probe was successfully applied to the ratiometric imaging of concentration dynamics of endogenously produced hydropersulfides in living cells.

Graphical abstract: Reversible ratiometric detection of highly reactive hydropersulfides using a FRET-based dual emission fluorescent probe

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Publication details

The article was received on 29 Aug 2016, accepted on 23 Sep 2016 and first published on 26 Sep 2016


Article type: Edge Article
DOI: 10.1039/C6SC03856E
Citation: Chem. Sci., 2017,8, 1134-1140
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    Reversible ratiometric detection of highly reactive hydropersulfides using a FRET-based dual emission fluorescent probe

    R. Kawagoe, I. Takashima, S. Uchinomiya and A. Ojida, Chem. Sci., 2017, 8, 1134
    DOI: 10.1039/C6SC03856E

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