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Issue 2, 2017
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A metal-mediated base pair that discriminates between the canonical pyrimidine nucleobases

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Abstract

A nucleoside analogue comprising the ligand 1H-imidazo[4,5-f][1,10]phenanthroline (P) was applied to develop a molecular beacon capable of discriminating the canonical nucleobases cytosine and thymine. The beacon is based on the formation of a stable Ag+-mediated base pair between P and cytosine, whereas the presence of Ag+ strongly destabilizes nucleic acids comprising an artificial base pair between P and thymine. Metal-mediated base pair formation was investigated by temperature-dependent UV spectroscopy and CD spectroscopy and complemented by extensive DFT calculations. The molecular beacon significantly extends the application spectrum of nucleic acids with metal-mediated base pairs. It is of potential use in the detection of single-nucleotide polymorphisms.

Graphical abstract: A metal-mediated base pair that discriminates between the canonical pyrimidine nucleobases

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Publication details

The article was received on 04 Aug 2016, accepted on 12 Oct 2016 and first published on 12 Oct 2016


Article type: Edge Article
DOI: 10.1039/C6SC03482A
Citation: Chem. Sci., 2017,8, 1337-1343
  • Open access: Creative Commons BY-NC license
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    A metal-mediated base pair that discriminates between the canonical pyrimidine nucleobases

    B. Jash, P. Scharf, N. Sandmann, C. Fonseca Guerra, D. A. Megger and J. Müller, Chem. Sci., 2017, 8, 1337
    DOI: 10.1039/C6SC03482A

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