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Issue 1, 2017
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MnO2 nanosheet mediated “DD–A” FRET binary probes for sensitive detection of intracellular mRNA

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Abstract

The donor donor–acceptor (DD–A) FRET model has proven to have a higher FRET efficiency than donor–acceptor acceptor (D–AA), donor–acceptor (D–A), and donor donor–acceptor acceptor (DD–AA) FRET models. The in-tube and in-cell experiments clearly demonstrate that the “DD–A” FRET binary probes can indeed increase the FRET efficiency and provide higher imaging contrast, which is about one order of magnitude higher than the ordinary “D–A” model. Furthermore, MnO2 nanosheets were employed to deliver these probes into living cells for intracellular TK1 mRNA detection because they can adsorb ssDNA probes, penetrate across the cell membrane and be reduced to Mn2+ ions by intracellular GSH. The results indicated that the MnO2 nanosheet mediated “DD–A” FRET binary probes are capable of sensitive and selective sensing gene expression and chemical-stimuli changes in gene expression levels in cancer cells. We believe that the MnO2 nanosheet mediated “DD–A” FRET binary probes have the potential as a simple but powerful tool for basic research and clinical diagnosis.

Graphical abstract: MnO2 nanosheet mediated “DD–A” FRET binary probes for sensitive detection of intracellular mRNA

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Publication details

The article was received on 18 Jul 2016, accepted on 01 Sep 2016 and first published on 05 Sep 2016


Article type: Edge Article
DOI: 10.1039/C6SC03162E
Citation: Chem. Sci., 2017,8, 668-673
  • Open access: Creative Commons BY-NC license
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    MnO2 nanosheet mediated “DD–A” FRET binary probes for sensitive detection of intracellular mRNA

    M. Ou, J. Huang, X. Yang, K. Quan, Y. Yang, N. Xie and K. Wang, Chem. Sci., 2017, 8, 668
    DOI: 10.1039/C6SC03162E

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