Jump to main content
Jump to site search

Issue 2, 2017
Previous Article Next Article

Engineering monolayer poration for rapid exfoliation of microbial membranes

Author affiliations

Abstract

The spread of bacterial resistance to traditional antibiotics continues to stimulate the search for alternative antimicrobial strategies. All forms of life, from bacteria to humans, are postulated to rely on a fundamental host defense mechanism, which exploits the formation of open pores in microbial phospholipid bilayers. Here we predict that transmembrane poration is not necessary for antimicrobial activity and reveal a distinct poration mechanism that targets the outer leaflet of phospholipid bilayers. Using a combination of molecular-scale and real-time imaging, spectroscopy and spectrometry approaches, we introduce a structural motif with a universal insertion mode in reconstituted membranes and live bacteria. We demonstrate that this motif rapidly assembles into monolayer pits that coalesce during progressive membrane exfoliation, leading to bacterial cell death within minutes. The findings offer a new physical basis for designing effective antibiotics.

Graphical abstract: Engineering monolayer poration for rapid exfoliation of microbial membranes

Back to tab navigation

Supplementary files

Publication details

The article was received on 03 Jul 2016, accepted on 25 Sep 2016 and first published on 26 Sep 2016


Article type: Edge Article
DOI: 10.1039/C6SC02925F
Citation: Chem. Sci., 2017,8, 1105-1115
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    Engineering monolayer poration for rapid exfoliation of microbial membranes

    A. Pyne, M. Pfeil, I. Bennett, J. Ravi, P. Iavicoli, B. Lamarre, A. Roethke, S. Ray, H. Jiang, A. Bella, B. Reisinger, D. Yin, B. Little, J. C. Muñoz-García, E. Cerasoli, P. J. Judge, N. Faruqui, L. Calzolai, A. Henrion, G. J. Martyna, C. R. M. Grovenor, J. Crain, B. W. Hoogenboom, A. Watts and M. G. Ryadnov, Chem. Sci., 2017, 8, 1105
    DOI: 10.1039/C6SC02925F

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements