Jump to main content
Jump to site search

Issue 1, 2017
Previous Article Next Article

Design and engineering of water-soluble light-harvesting protein maquettes

Author affiliations

Abstract

Natural selection in photosynthesis has engineered tetrapyrrole based, nanometer scale, light harvesting and energy capture in light-induced charge separation. By designing and creating nanometer scale artificial light harvesting and charge separating proteins, we have the opportunity to reengineer and overcome the limitations of natural selection to extend energy capture to new wavelengths and to tailor efficient systems that better meet human as opposed to cellular energetic needs. While tetrapyrrole cofactor incorporation in natural proteins is complex and often assisted by accessory proteins for cofactor transport and insertion, artificial protein functionalization relies on a practical understanding of the basic physical chemistry of protein and cofactors that drive nanometer scale self-assembly. Patterning and balancing of hydrophobic and hydrophilic tetrapyrrole substituents is critical to avoid natural or synthetic porphyrin and chlorin aggregation in aqueous media and speed cofactor partitioning into the non-polar core of a man-made water soluble protein designed according to elementary first principles of protein folding. This partitioning is followed by site-specific anchoring of tetrapyrroles to histidine ligands strategically placed for design control of rates and efficiencies of light energy and electron transfer while orienting at least one polar group towards the aqueous phase.

Graphical abstract: Design and engineering of water-soluble light-harvesting protein maquettes

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 01 Jun 2016, accepted on 16 Aug 2016 and first published on 17 Aug 2016


Article type: Edge Article
DOI: 10.1039/C6SC02417C
Citation: Chem. Sci., 2017,8, 316-324
  • Open access: Creative Commons BY license
  •   Request permissions

    Design and engineering of water-soluble light-harvesting protein maquettes

    G. Kodali, J. A. Mancini, L. A. Solomon, T. V. Episova, N. Roach, C. J. Hobbs, P. Wagner, O. A. Mass, K. Aravindu, J. E. Barnsley, K. C. Gordon, D. L. Officer, P. L. Dutton and C. C. Moser, Chem. Sci., 2017, 8, 316
    DOI: 10.1039/C6SC02417C

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author