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Issue 1, 2017
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Selective detection of N6-methyladenine in DNA via metal ion-mediated replication and rolling circle amplification

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Abstract

N6-methyladenine (6mA) is reported as a potential epigenetic marker in eukaryotic genomes. However, accurate identification of the location of 6mA in DNA remains a challenging task. Here, we show that Ag+ can selectively stabilize the A–C mismatch and efficiently promote primer extension. In contrast, the complex of 6mA–Ag+–C is instable and therefore cannot be recognized by DNA polymerases, resulting in the termination of primer extension. Based on this finding, we successfully identified and quantified 6mA at the single-base level through the analysis of gel bands of extended primers and fluorescence measurements combined with rolling circle amplification. The high selectivity and sensitivity of this strategy may provide a new platform for the efficient analysis of 6mA in DNA in the future.

Graphical abstract: Selective detection of N6-methyladenine in DNA via metal ion-mediated replication and rolling circle amplification

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Publication details

The article was received on 22 May 2016, accepted on 09 Aug 2016 and first published on 10 Aug 2016


Article type: Edge Article
DOI: 10.1039/C6SC02271E
Citation: Chem. Sci., 2017,8, 200-205
  • Open access: Creative Commons BY license
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    Selective detection of N6-methyladenine in DNA via metal ion-mediated replication and rolling circle amplification

    T. Hong, Y. Yuan, T. Wang, J. Ma, Q. Yao, X. Hua, Y. Xia and X. Zhou, Chem. Sci., 2017, 8, 200
    DOI: 10.1039/C6SC02271E

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