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Issue 102, 2016, Issue in Progress
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Membrane permeabilization by conjugated oligoelectrolytes accelerates whole-cell catalysis

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Abstract

Conjugated oligoelectrolytes (COEs) boost the electrical performance of a wide range of bioelectrochemical systems, yet their mechanism of action remains incompletely understood. One possible mode of action is that COEs permeabilize the cell envelope. We thus examined the effect of tetracationic COE, DSSN+, on the permeability of the inner and outer membrane of Escherichia coli by detecting extracellular activity of normally periplasmic and cytoplasmic enzymes. DSSN+ increases the release of the periplasmic enzyme alkaline phosphatase (ALP) up to 20-fold, but does not significantly change the release of the cytoplasmic enzyme β-galactosidase. Additionally, DSSN+ caused a 2-fold increase in the turnover of a cytoplasmic substrate. These studies present a more complete understanding of the mechanism of action in bioelectrochemical systems and pivot future applications of COEs towards a method for improving whole-cell catalysis.

Graphical abstract: Membrane permeabilization by conjugated oligoelectrolytes accelerates whole-cell catalysis

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Publication details

The article was received on 16 Sep 2016, accepted on 10 Oct 2016 and first published on 14 Oct 2016


Article type: Paper
DOI: 10.1039/C6RA23083K
Citation: RSC Adv., 2016,6, 100300-100306
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    Membrane permeabilization by conjugated oligoelectrolytes accelerates whole-cell catalysis

    C. Catania, Caroline M. Ajo-Franklin and G. C. Bazan, RSC Adv., 2016, 6, 100300
    DOI: 10.1039/C6RA23083K

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