Jump to main content
Jump to site search

Issue 99, 2016, Issue in Progress
Previous Article Next Article

Gene delivery using dendrimer/pDNA complexes immobilized in electrospun fibers using the Layer-by-Layer technique

Author affiliations

Abstract

A gene delivery platform for potential use in tissue engineering applications was developed by surface functionalization of biodegradable electrospun poly(lactic-co-glycolic acid) (PLGA) fibers with nanolayers of chitosan (cationic polymer) and alginate (anionic polymer) using the Layer-by-Layer (LbL) technique. The developed system not only supported the attachment and growth of human Mesenchymal Stem Cells (hMSCs), but also was capable of delivering pDNA/dendrimer complexes and inducing cell differentiation towards the osteogenic lineage when a pDNA codifying for human Bone Morphogenetic Protein-2 (BMP-2) was used. Beyond providing a means for pDNA/dendrimer complex immobilization, the polyelectrolyte coating conferred sustained release properties to the scaffold that resulted in pDNA protection from degradation. The polyelectrolyte coating, by itself, also contributed to enhance cell differentiation.

Graphical abstract: Gene delivery using dendrimer/pDNA complexes immobilized in electrospun fibers using the Layer-by-Layer technique

Back to tab navigation

Publication details

The article was received on 07 Sep 2016, accepted on 01 Oct 2016 and first published on 05 Oct 2016


Article type: Paper
DOI: 10.1039/C6RA22444J
Citation: RSC Adv., 2016,6, 97116-97128
  •   Request permissions

    Gene delivery using dendrimer/pDNA complexes immobilized in electrospun fibers using the Layer-by-Layer technique

    K. Ramalingam, R. Castro, P. Pires, X. Shi, J. Rodrigues, S. Xiao and H. Tomás, RSC Adv., 2016, 6, 97116
    DOI: 10.1039/C6RA22444J

Search articles by author

Spotlight

Advertisements