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Issue 103, 2016
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An enzyme-mediated in situ hydrogel based on polyaspartamide derivatives for localized drug delivery and 3D scaffolds

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Abstract

An injectable and biodegradable enzymatically crosslinked hydrogel based on poly(α,β-aspartic acid) modified with tyramine (TA) and 3-amino-1-propanol (AP) (PASP-g-TA/AP) was prepared under physiological conditions in the presence of horseradish peroxidase (HRP) and hydrogen peroxide (H2O2). The polymer PASP-g-TA/AP was readily synthesized through aminolysis of polysuccinimide (PSI) with TA and subsequently with AP. The gelation time could be tuned from 10 s to 8 min by modulating the concentrations of polymer, HRP and H2O2 cooperatively. Studies on swelling ratio, hydrogel morphology and a rheology analysis further demonstrated the relationship between the crosslinking density and the concentrations of the polymer, HRP and H2O2. The PASP-g-TA/AP hydrogels displayed a considerable capacity to control the release of hydrophilic small model drugs at higher polymer concentrations. The cell culture study showed that COS-7 cells exhibited high viability in vitro and notable proliferation when cultured within the PASP-g-TA/AP hydrogel. After subcutaneous injection into mice, the PASP-g-TA/AP hydrogels formed rapidly in situ and persisted for up to 30 days with an acceptable inflammatory response. These results suggest that the newly described PASP-g-TA/AP hydrogels have good biocompatibility and are promising biomaterials as targeted drug delivery carriers and three-dimensional scaffolds.

Graphical abstract: An enzyme-mediated in situ hydrogel based on polyaspartamide derivatives for localized drug delivery and 3D scaffolds

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Publication details

The article was received on 20 Jul 2016, accepted on 11 Oct 2016 and first published on 11 Oct 2016


Article type: Paper
DOI: 10.1039/C6RA18479K
Citation: RSC Adv., 2016,6, 101334-101346
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    An enzyme-mediated in situ hydrogel based on polyaspartamide derivatives for localized drug delivery and 3D scaffolds

    X. Cheng, J. Liu, L. Wang, R. Wang, Z. Liu and R. Zhuo, RSC Adv., 2016, 6, 101334
    DOI: 10.1039/C6RA18479K

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