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Issue 112, 2016
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Protein elicitor isolated from Escherichia coli induced bioactive compound biosynthesis as well as gene expression in Glycyrrhiza uralensis Fisch adventitious roots

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Abstract

This study explored the ability of three rhizobacterial strains (Bacillus subtilis, Penicillium fellutanum and Escherichia coli) to trigger metabolism. The protein fragment of more than 10 kDa significantly increased the metabolite contents in Glycyrrhiza uralensis adventitious roots. The results showed the highest accumulation of total flavonoids (7.59 mg g−1), glycyrrhizic acid (0.29 mg g−1), glycyrrhetinic acid (0.27 mg g−1) and polysaccharide (93.11 mg g−1) by up to 2.27-fold, 2.64-fold, 2.70-fold and 2.32-fold that of control roots, respectively. Besides, the protein fragment of more than 10 kDa significantly activated defense signaling and extremely up-regulated the expression of defense-related genes and functional genes in glycyrrhizic acid and flavonoid biosynthesis. In Glycyrrhiza uralensis adventitious roots, HPLC-ESI-MSn analysis showed that the protein fragment of more than 10 kDa induced the generation of four new compounds over the control group.

Graphical abstract: Protein elicitor isolated from Escherichia coli induced bioactive compound biosynthesis as well as gene expression in Glycyrrhiza uralensis Fisch adventitious roots

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Publication details

The article was received on 01 Jul 2016, accepted on 13 Oct 2016 and first published on 19 Oct 2016


Article type: Paper
DOI: 10.1039/C6RA16903A
Citation: RSC Adv., 2016,6, 111622-111631
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    Protein elicitor isolated from Escherichia coli induced bioactive compound biosynthesis as well as gene expression in Glycyrrhiza uralensis Fisch adventitious roots

    J. Li, J. Wang, J. Li, J. Li, S. Liu and W. Gao, RSC Adv., 2016, 6, 111622
    DOI: 10.1039/C6RA16903A

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