Supported lipid bilayer membrane arrays on micro-patterned ITO electrodes

Abstract

Lipid bilayer arrays were formed on ITO electrodes coated with a patterned self-assembled trimethoxy(octadecyl)silane monolayer (TODS-SAM). The TODS-SAM was patterned by deep UV (254 nm) irradiation through a quartz mask to create the patterned SAM modified ITO surface. Field-emission scanning electron microscopy was applied to characterize the patterned TODS SAM modified ITO. The lipid bilayer arrays were characterized by fluorescence microscopy, cyclic voltammetry and electrochemical impedance spectroscopy. With this bilayer array platform both the fluorescence microscopy and electrochemical detection can be realized to explore the biophysical properties of the cell membrane.