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Issue 44, 2016
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Protein storage with perfluorinated PEG compartments in a hydrofluorocarbon solvent

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Abstract

We report a novel storage technology of proteins with surface-perfluorinated poly(ethylene glycol) compartments in 2H,3H-perfluoropentane. The compartments were obtained from self-folding and self-assembly of an amphiphilic random copolymer bearing poly(ethylene glycol) and perfluoroalkyl pendants in the hydrofluorocarbon. Lysozyme and α-chymotrypsin as model proteins were efficiently encapsulated within the PEG compartments and quantitatively recovered therefrom with water. The recovered lysozyme maintained the original higher order structure without denaturation to show enzymatic activity for the hydrolysis of Micrococcus lysodeikticus as high as its original counterpart in water. The storage technology was further effective to inhibit inactivation of α-chymotrypsin.

Graphical abstract: Protein storage with perfluorinated PEG compartments in a hydrofluorocarbon solvent

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Publication details

The article was received on 30 Jul 2016, accepted on 31 Aug 2016 and first published on 01 Sep 2016


Article type: Communication
DOI: 10.1039/C6PY01333C
Citation: Polym. Chem., 2016,7, 6694-6698
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    Protein storage with perfluorinated PEG compartments in a hydrofluorocarbon solvent

    Y. Koda, T. Terashima, H. D. Maynard and M. Sawamoto, Polym. Chem., 2016, 7, 6694
    DOI: 10.1039/C6PY01333C

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