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Issue 11, 2016
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Complex formation of albumin with tricarbocyanine dyes containing phosphonate groups

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Abstract

The spectral characteristics, binding constants with bovine (BSA) and human serum albumin (HSA) and lifetimes of fluorescence in PBS and EtOH solutions and in the presence of BSA in PBS were measured for novel indotricarbocyanine dyes bearing remote phosphonate groups. These parameters are close to those for indocyanine green (ICG) indicating that the Coulomb interaction does not play a significant role in complex formation, and the binding is determined by the interaction of the dye polymethine chain with albumin. The fluorescence lifetimes of the complexes with BSA strongly indicate the formation of complexes of two types with different lifetimes. The complex with a longer fluorescence lifetime (740–800 ps) and major contribution (up to 88%) is bound to the more hydrophobic site and that with a shorter fluorescence lifetime (300–340 ps) to the more hydrophilic site.

Graphical abstract: Complex formation of albumin with tricarbocyanine dyes containing phosphonate groups

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Publication details

The article was received on 05 Jul 2016, accepted on 18 Sep 2016 and first published on 19 Sep 2016


Article type: Paper
DOI: 10.1039/C6PP00246C
Citation: Photochem. Photobiol. Sci., 2016,15, 1377-1384
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    Complex formation of albumin with tricarbocyanine dyes containing phosphonate groups

    V. A. Kuzmin, T. D. Nekipelova, T. A. Podrugina, G. V. Golovina, A. A. Kostyukov, V. V. Temnov, I. A. Doroshenko, E. V. Radchenko, V. A. Palyulin and N. S. Zefirov, Photochem. Photobiol. Sci., 2016, 15, 1377
    DOI: 10.1039/C6PP00246C

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