Issue 17, 2016
From the journal:

Organic & Biomolecular Chemistry

Nitroxide amide-BODIPY probe behavior in fibroblasts analyzed by advanced fluorescence microscopy†

M. Liras,‡ab   S. Simoncelli,§bc   A. Rivas-Aravena,d   O. García, ORCID logo a   J. C. Scaiano,b   E. I. Alarcon*be  and  A. Aspée*f  

* Corresponding authors

a Instituto de Ciencia y Tecnología de Polímeros, Consejo Superior de Investigaciones Científicas (ICTP-CSIC), Juan de la Cierva 3, Madrid, España

b Department of Chemistry and Biomolecular Sciences and Centre for Catalysis Research and Innovation, University of Ottawa, 10 Marie Curie, Ottawa, Ontario K1N6N5, Canada

c INQUIMAE and Departamento de Química Inorgánica, Analítica, y Química Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina

d Laboratorio de Radiobiología Molecular y Celular, Departamento de Aplicaciones Nucleares, Comisión Chilena de Energía Nuclear, Santiago, Chile

e Bio-nanomaterials Chemistry and Engineering Laboratory, Division of Cardiac Surgery, University of Ottawa Heart Institute, 40 Ruskin St., Ottawa, Ontario K1Y 4W7, Canada
E-mail: ealarcon@ottawa.heart.ca

f Facultad de Química y Biología, Universidad de Santiago de Chile, Casilla 40 Correo 33, Santiago, Chile
E-mail: alexis.aspee@usach.cl

Abstract

A novel synthesized nitroxide amide-BODIPY prefluorescent probe was used to study cellular redox balance that modulates nitroxide/hydroxylamine ratio in cultured human fibroblasts. FLIM quantitatively differentiated between nitroxide states of the cytoplasm-localized probe imaged by TIRF, monitoring nitroxide depletion by hydrogen peroxide; eluding incorrect interpretation if only fluorescence intensity is considered.

Graphical abstract: Nitroxide amide-BODIPY probe behavior in fibroblasts analyzed by advanced fluorescence microscopy

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Article information

DOI
https://doi.org/10.1039/C6OB00533K
Article type
Communication
Submitted
10 Mar 2016
Accepted
01 Apr 2016
First published
04 Apr 2016

Org. Biomol. Chem., 2016,14, 4023-4026

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Nitroxide amide-BODIPY probe behavior in fibroblasts analyzed by advanced fluorescence microscopy

M. Liras, S. Simoncelli, A. Rivas-Aravena, O. García, J. C. Scaiano, E. I. Alarcon and A. Aspée, Org. Biomol. Chem., 2016, 14, 4023 DOI: 10.1039/C6OB00533K

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