Issue 9, 2016

Inhibition of HL-60 cell growth via cell cycle arrest and apoptosis induction by a cycloartane–labdane heterodimer from Pseudolarix amabilis

Abstract

Pseudolaridimer C (1), a rarely encountered cycloartane–labdane Diels–Alder adduct was isolated from the cones of Pseudolarix amabilis. The structure and absolute configuration of 1 were established by comprehensive NMR and CD spectral analysis. The WST-8 assay indicated that 1 time and dose dependently inhibited the proliferation of human leukemia cells HL-60 at 1–10 μM. DAPI and Annexin V-FITC/PI double staining method, and DNA ladder experiments all proved that 1 had significant dose-dependent effects on HL-60 cell apoptosis. A further mechanism study indicated that the apoptosis was associated with the cell cycle arrest during the G2/M phase, and the activation of caspase-9, -3, -7, and poly-(ADP-ribose)-polymerase (PARP).

Graphical abstract: Inhibition of HL-60 cell growth via cell cycle arrest and apoptosis induction by a cycloartane–labdane heterodimer from Pseudolarix amabilis

Supplementary files

Article information

Article type
Paper
Submitted
28 Dec 2015
Accepted
26 Jan 2016
First published
26 Jan 2016

Org. Biomol. Chem., 2016,14, 2618-2624

Inhibition of HL-60 cell growth via cell cycle arrest and apoptosis induction by a cycloartane–labdane heterodimer from Pseudolarix amabilis

X. Tian, N. Yang, B. Li, J. Zhang, X. Xu, R. Yue, H. Li, L. Chen, Y. Shen and W. Zhang, Org. Biomol. Chem., 2016, 14, 2618 DOI: 10.1039/C5OB02661J

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