Issue 9, 2016

Hybrid phase ligation for efficient synthesis of histone proteins

Abstract

We introduce a hybrid solid-solution phase ligation approach that combines the efficiency of solid phase ligation with solution phase ligation in the total synthesis of modified histone proteins. A two linker strategy allows analysis throughout work on the solid phase and maximizes yields through cleavage at an external Rink, while an internal HMBA linker allows the native carboxyl terminus for any protein sequence. We demonstrate this approach for two histone proteins: triple-acetylated H4-K5ac, K12ac, K91ac and CENP-A-K124ac.

Graphical abstract: Hybrid phase ligation for efficient synthesis of histone proteins

Supplementary files

Article information

Article type
Communication
Submitted
23 Oct 2015
Accepted
15 Jan 2016
First published
20 Jan 2016

Org. Biomol. Chem., 2016,14, 2603-2607

Author version available

Hybrid phase ligation for efficient synthesis of histone proteins

R. R. Yu, S. K. Mahto, K. Justus, M. M. Alexander, C. J. Howard and J. J. Ottesen, Org. Biomol. Chem., 2016, 14, 2603 DOI: 10.1039/C5OB02195B

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