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Issue 42, 2016
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Vertical nanocolumn-assisted pluripotent stem cell colony formation with minimal cell-penetration

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Abstract

The biological applications of vertical nanostructures mostly rely on their intracellular accessibility through the cellular membrane by promoting cell-to-nanostructure interactions. Herein, we report a seemingly counter-intuitive approach for the spontaneous formation of mouse induced pluripotent stem cell (iPSC)-derived three-dimensional spherical colonies with unlimited self-renewal and differentiation potential. The comprehensive analyses of iPSCs cultured on vertical silicon nanocolumn arrays (vSNAs) with various nanocolumn geometries show reduced cell-to-substrate adhesion and enhanced cell-to-cell interactions under optimized vSNA conditions, successfully accommodating the spontaneous production of iPSC-derived spherical colonies. Remarkably, these colonies which were only minimally penetrated by and thereby easily harvested from wafer-sized vSNAs display a substantial increase in pluripotency marker expression and successfully differentiate into three germ layers. Our vSNAs capable of large-scale fabrication, efficient for spherical colony formation, and reusable for multiple iPSC culture could serve as a broad-impact culture platform for stem cell research.

Graphical abstract: Vertical nanocolumn-assisted pluripotent stem cell colony formation with minimal cell-penetration

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Publication details

The article was received on 05 Aug 2016, accepted on 14 Sep 2016 and first published on 16 Sep 2016


Article type: Paper
DOI: 10.1039/C6NR06203B
Citation: Nanoscale, 2016,8, 18087-18097
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    Vertical nanocolumn-assisted pluripotent stem cell colony formation with minimal cell-penetration

    H. Kim, D. H. Kang, K. H. Koo, S. Lee, S. Kim, J. Kim, M. Yoon, S. Y. Kim and E. G. Yang, Nanoscale, 2016, 8, 18087
    DOI: 10.1039/C6NR06203B

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