Issue 36, 2016
From the journal:

Nanoscale

Direct real-time detection of single proteins using silicon nanowire-based electrical circuits†

Jie Li,ab   Gen He,ab   Hiroshi Ueno,c   Chuancheng Jia,b   Hiroyuki Noji,*c   Chuanmin Qi*a  and  Xuefeng Guo*bd  

* Corresponding authors

a Key Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing 100875, P. R. China
E-mail: qichuanmin@bnu.edu.cn

b Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China
E-mail: guoxf@pku.edu.cn

c Department of Applied Chemistry, School of Engineering, The University of Tokyo, Tokyo 113-8654, Japan
E-mail: hnoji@appchem.t.u-tokyo.ac.jp

d Department of Materials Science and Engineering, College of Engineering, Peking University, Beijing 100871, P. R. China

Abstract

We present an efficient strategy through surface functionalization to build a single silicon nanowire field-effect transistor-based biosensor that is capable of directly detecting protein adsorption/desorption at the single-event level. The step-wise signals in real-time detection of His-tag F1-ATPases demonstrate a promising electrical biosensing approach with single-molecule sensitivity, thus opening up new opportunities for studying single-molecule biophysics in broad biological systems.

Graphical abstract: Direct real-time detection of single proteins using silicon nanowire-based electrical circuits

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Article information

DOI
https://doi.org/10.1039/C6NR04103E
Article type
Communication
Submitted
20 May 2016
Accepted
18 Aug 2016
First published
19 Aug 2016

Nanoscale, 2016,8, 16172-16176

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Direct real-time detection of single proteins using silicon nanowire-based electrical circuits

J. Li, G. He, H. Ueno, C. Jia, H. Noji, C. Qi and X. Guo, Nanoscale, 2016, 8, 16172 DOI: 10.1039/C6NR04103E

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