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Issue 11, 2016
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Peroxidase-like activity of the Co3O4 nanoparticles used for biodetection and evaluation of antioxidant behavior

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Abstract

Nanostructured enzyme mimics are of great interest as promising alternatives to artificial enzymes for biomedical and catalytic applications. Studying the chemical interactions between antioxidants and nano-enzymes may result in a better understanding of the antioxidant capability of antioxidants and may help improve the function of artificial enzymes to better mimic natural enzymes. In this study, using Co3O4 nanoparticles (NPs) as peroxidase mimics to catalyze the oxidation of chromophoric substrates by H2O2, we developed a platform that acts as a biosensor for hydrogen peroxide and glucose and that can study the inhibitory effects of natural antioxidants on peroxidase mimics. This method can be applied specifically to glucose detection in real samples. Three natural antioxidants, gallic acid (GA), tannic acid (TA), and ascorbic acid (AA), were compared for their antioxidant capabilities. We found that these three antioxidants efficiently inhibit peroxidase-like activity with concentration dependence. The antioxidants showed different efficiencies, in the following order: tannic acid > gallic acid > ascorbic acid. They also showed distinct modes of inhibition based on different interaction mechanisms. This study serves as a proof-of-concept that nano-enzyme mimics can be used to evaluate antioxidant capabilities and to screen enzyme inhibitors.

Graphical abstract: Peroxidase-like activity of the Co3O4 nanoparticles used for biodetection and evaluation of antioxidant behavior

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Publication details

The article was received on 30 Jan 2016, accepted on 12 Feb 2016 and first published on 16 Feb 2016


Article type: Paper
DOI: 10.1039/C6NR00860G
Citation: Nanoscale, 2016,8, 5938-5945
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    Peroxidase-like activity of the Co3O4 nanoparticles used for biodetection and evaluation of antioxidant behavior

    H. Jia, D. Yang, X. Han, J. Cai, H. Liu and W. He, Nanoscale, 2016, 8, 5938
    DOI: 10.1039/C6NR00860G

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