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Issue 20, 2016
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Engineering of supramolecular photoactive protein architectures: the defined co-assembly of photosystem I and cytochrome c using a nanoscaled DNA-matrix

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Abstract

The engineering of renewable and sustainable protein-based light-to-energy converting systems is an emerging field of research. Here, we report on the development of supramolecular light-harvesting electrodes, consisting of the redox protein cytochrome c working as a molecular scaffold as well as a conductive wiring network and photosystem I as a photo-functional matrix element. Both proteins form complexes in solution, which in turn can be adsorbed on thiol-modified gold electrodes through a self-assembly mechanism. To overcome the limited stability of self-grown assemblies, DNA, a natural polyelectrolyte, is used as a further building block for the construction of a photo-active 3D architecture. DNA acts as a structural matrix element holding larger protein amounts and thus remarkably improving the maximum photocurrent and electrode stability. On investigating the photophysical properties, this system demonstrates that effective electron pathways have been created.

Graphical abstract: Engineering of supramolecular photoactive protein architectures: the defined co-assembly of photosystem I and cytochrome c using a nanoscaled DNA-matrix

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Publication details

The article was received on 05 Jan 2016, accepted on 15 Apr 2016 and first published on 15 Apr 2016


Article type: Paper
DOI: 10.1039/C6NR00097E
Author version available: Download Author version (PDF)
Citation: Nanoscale, 2016,8, 10695-10705
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    Engineering of supramolecular photoactive protein architectures: the defined co-assembly of photosystem I and cytochrome c using a nanoscaled DNA-matrix

    K. R. Stieger, D. Ciornii, A. Kölsch, M. Hejazi, H. Lokstein, S. C. Feifel, A. Zouni and F. Lisdat, Nanoscale, 2016, 8, 10695
    DOI: 10.1039/C6NR00097E

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