Issue 24, 2016

Plasmonic gratings with nano-protrusions made by glancing angle deposition for single-molecule super-resolution imaging

Abstract

Super-resolution imaging has been advantageous in studying biological and chemical systems, but the required equipment and platforms are expensive and unable to observe single-molecules at the high (μM) fluorophore concentrations required to study protein interaction and enzymatic activity. Here, a plasmonic platform was designed that utilized an inexpensively fabricated plasmonic grating in combination with a scalable glancing angle deposition (GLAD) technique using physical vapor deposition. The GLAD creates an abundance of plasmonic nano-protrusion probes that combine the surface plasmon resonance (SPR) from the periodic gratings with the localized SPR of these nano-protrusions. The resulting platform enables simultaneous imaging of a large area without point-by-point scanning or bulk averaging for the detection of single Cyanine-5 molecules in dye concentrations ranging from 50 pM to 10 μM using epifluorescence microscopy. Combining the near-field plasmonic nano-protrusion probes and super-resolution technique using localization microscopy, we demonstrate the ability to resolve grain sizes down to 65 nm. This plasmonic GLAD grating is a cost-effective super-resolution imaging substrate with potential applications in high-speed biomedical imaging over a wide range of fluorescent concentrations.

Graphical abstract: Plasmonic gratings with nano-protrusions made by glancing angle deposition for single-molecule super-resolution imaging

Supplementary files

Article information

Article type
Paper
Submitted
23 Dec 2015
Accepted
16 May 2016
First published
24 May 2016

Nanoscale, 2016,8, 12189-12201

Plasmonic gratings with nano-protrusions made by glancing angle deposition for single-molecule super-resolution imaging

B. Chen, A. Wood, A. Pathak, J. Mathai, S. Bok, H. Zheng, S. Hamm, S. Basuray, S. Grant, K. Gangopadhyay, P. V. Cornish and S. Gangopadhyay, Nanoscale, 2016, 8, 12189 DOI: 10.1039/C5NR09165A

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