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Issue 1, 2016
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Prospecting for new bacterial metabolites: a glossary of approaches for inducing, activating and upregulating the biosynthesis of bacterial cryptic or silent natural products

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Abstract

Covering: up to 2015

Over the centuries, microbial secondary metabolites have played a central role in the treatment of human diseases and have revolutionised the pharmaceutical industry. With the increasing number of sequenced microbial genomes revealing a plethora of novel biosynthetic genes, natural product drug discovery is entering an exciting second golden age. Here, we provide a concise overview as an introductory guide to the main methods employed to unlock or up-regulate these so called ‘cryptic’, ‘silent’ and ‘orphan’ gene clusters, and increase the production of the encoded natural product. With a predominant focus on bacterial natural products we will discuss the importance of the bioinformatics approach for genome mining, the use of first different and simple culturing techniques and then the application of genetic engineering to unlock the microbial treasure trove.

Graphical abstract: Prospecting for new bacterial metabolites: a glossary of approaches for inducing, activating and upregulating the biosynthesis of bacterial cryptic or silent natural products

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Publication details

The article was received on 09 Sep 2015 and first published on 05 Nov 2015


Article type: Review Article
DOI: 10.1039/C5NP00111K
Citation: Nat. Prod. Rep., 2016,33, 54-72
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    Prospecting for new bacterial metabolites: a glossary of approaches for inducing, activating and upregulating the biosynthesis of bacterial cryptic or silent natural products

    J. S. Zarins-Tutt, T. T. Barberi, H. Gao, A. Mearns-Spragg, L. Zhang, D. J. Newman and R. J. M. Goss, Nat. Prod. Rep., 2016, 33, 54
    DOI: 10.1039/C5NP00111K

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