Jump to main content
Jump to site search

Issue 11, 2016
Previous Article Next Article

Investigation of uranium interactions with calcium phosphate-binding proteins using ICP/MS and CE-ICP/MS

Author affiliations

Abstract

During long-term exposure, uranium accumulates in bone. Since uranium in U(VI) complexes shares similar coordination properties to calcium, this toxicant is assumed to be exchanged with calcium ions at the surfaces of bone mineral crystals. Recently, two proteins involved in bone turnover, fetuin A and osteopontin, were shown to exhibit a high affinity for U(VI). A common biochemical feature of both fetuin A and osteopontin is their inhibiting role in calcium phosphate precipitation. Therefore it is conceivable that complexation of U(VI) with these proteins may alter their interaction with calcium and/or calcium phosphate. Quantitative analyses of calcium, phosphorus and uranium performed using inductively coupled plasma/mass spectrometry (ICP/MS) demonstrated the inhibition of the precipitation of calcium phosphate by fetuin A and osteopontin for 2 h. In addition, the presence of U(VI) did not seem to alter the duration of this process. However, dynamic light scattering studies revealed that the size of the colloidal particles formed with osteopontin was altered by the presence of U(VI) in a concentration-dependent manner. Finally, using hyphenated capillary electrophoresis-ICP/MS (CE-ICP/MS), we showed that in these systems, at a low concentration of U(VI) (protein : U(VI) 8 : 1), U(VI) might remain in solution by forming a complex with proteins and not by sequestration of a precipitate of either autunite or uranyl orthophosphate.

Graphical abstract: Investigation of uranium interactions with calcium phosphate-binding proteins using ICP/MS and CE-ICP/MS

Back to tab navigation

Publication details

The article was received on 29 Jun 2016, accepted on 14 Sep 2016 and first published on 26 Sep 2016


Article type: Paper
DOI: 10.1039/C6MT00147E
Citation: Metallomics, 2016,8, 1185-1192
  •   Request permissions

    Investigation of uranium interactions with calcium phosphate-binding proteins using ICP/MS and CE-ICP/MS

    T. S. Huynh, C. Vidaud and A. Hagège, Metallomics, 2016, 8, 1185
    DOI: 10.1039/C6MT00147E

Search articles by author

Spotlight

Advertisements