Jump to main content
Jump to site search

Issue 9, 2016
Previous Article Next Article

Copper-responsive gene expression in the methanotroph Methylosinus trichosporium OB3b

Author affiliations

Abstract

Methanotrophic bacteria convert methane to methanol using methane monooxygenase (MMO) enzymes. In many strains, either an iron-containing soluble (sMMO) or a copper-containing particulate (pMMO) enzyme can be produced depending on copper availability; the mechanism of this copper switch has not been elucidated. A key player in methanotroph copper homeostasis is methanobactin (Mbn), a ribosomally produced, post-translationally modified natural product with a high affinity for copper. The Mbn precursor peptide is encoded within an operon that contains a range of putative transporters, regulators, and biosynthetic proteins, but the involvement of these genes in Mbn-related processes remains unclear. Extensive time-dependent qRT-PCR studies of Methylosinus trichosporium OB3b and the constitutive sMMO-producing mutant M. trichosporium OB3b PP358 show that the Mbn operon is indeed copper-regulated, providing experimental support for its bioinformatics-based identification. Moreover, the Mbn operon is co-regulated with the sMMO operon and reciprocally regulated with the pMMO operon. Within the Mbn and sMMO operons, a subset of regulatory genes exhibits a distinct and shared pattern of expression, consistent with their proposed functions as internal regulators. In addition, genome sequencing of the M. trichosporium OB3b PP358 mutant provides new evidence for the involvement of genes adjacent to the pMMO operon in methanotroph copper homeostasis.

Graphical abstract: Copper-responsive gene expression in the methanotroph Methylosinus trichosporium OB3b

Back to tab navigation

Supplementary files

Publication details

The article was received on 09 Nov 2015, accepted on 01 Apr 2016 and first published on 07 Apr 2016


Article type: Paper
DOI: 10.1039/C5MT00289C
Author version available: Download Author version (PDF)
Citation: Metallomics, 2016,8, 931-940
  •   Request permissions

    Copper-responsive gene expression in the methanotroph Methylosinus trichosporium OB3b

    G. E. Kenney, M. Sadek and A. C. Rosenzweig, Metallomics, 2016, 8, 931
    DOI: 10.1039/C5MT00289C

Search articles by author

Spotlight

Advertisements