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Issue 3, 2016
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Cellular uptake of metallated cobalamins

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Abstract

Cellular uptake of vitamin B12-cisplatin conjugates was estimated via detection of their metal constituents (Co, Pt, and Re) by inductively coupled plasma mass spectrometry (ICP-MS). Vitamin B12 (cyano-cob(III)alamin) and aquo-cob(III)alamin [Cbl-OH2]+, which differ in the β-axial ligands (CN and H2O, respectively), were included as control samples. The results indicated that B12 derivatives delivered cisplatin to both cellular cytosol and nuclei with an efficiency of one third compared to the uptake of free cisplatin cis-[PtIICl2(NH3)2]. In addition, uptake of charged B12 derivatives including [Cbl-OH2]+, [{Co}-CN-{cis-PtCl(NH3)2}]+, [{Re}-{Co}-CN-{cis-PtCl(NH3)2}]+, and [{Co}-CN-{trans-Pt(Cyt)(NH3)2}]2+ (Cyt = cytarabin) was high compared to neutral B12, which implied the existence of an additional internalization pathway for charged B12 vitamin analogs. The affinities of the charged B12 derivatives to the B12 transporters HC, IF and TC were similar to that of native vitamin B12.

Graphical abstract: Cellular uptake of metallated cobalamins

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Publication details

The article was received on 21 Oct 2015, accepted on 22 Dec 2015 and first published on 22 Dec 2015


Article type: Paper
DOI: 10.1039/C5MT00272A
Citation: Metallomics, 2016,8, 298-304
  • Open access: Creative Commons BY license
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    Cellular uptake of metallated cobalamins

    M. T. Q. Tran, S. Stürup, I. H. Lambert, B. Gammelgaard, E. Furger and R. Alberto, Metallomics, 2016, 8, 298
    DOI: 10.1039/C5MT00272A

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