Target-selective photo-degradation of a sialyl Lewis a (sLea) conjugate and photo-cytotoxicity against sLea positive cancer cells using an anthraquinone-antibody hybrid

Abstract

A purpose-designed anthraquinone–monoclonal antibody (anti-sialyl Lewis A (sLe^a) mAb) hybrid 6 selectively bound to and effectively degraded the target glycoprotein, HSA (human serum albumin)–sLe^a conjugate 4. Degradation was achieved using long wavelength UV light irradiation in the absence of any additives and under neutral conditions. Furthermore, the hybrid 6 exhibited selective photo-cytotoxicity against the sLe^a positive cancer cells A431 and WiDr cells only upon photo-irradiation.