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Issue 12, 2016
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Glycomic profiling of targeted serum haptoglobin for gastric cancer using nano LC/MS and LC/MS/MS

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Abstract

Gastric cancer has one of the highest cancer mortality rates worldwide, largely because of difficulties in early-stage detection. Aberrant glycosylation in serum proteins is associated with many human diseases including inflammation and various types of cancer. Serum-based global glycan profiling using mass spectrometry has been explored and has already led to several potential glycan markers for several disease states. However, localization of the aberrant glycosylation is desirable in order to improve the specificity and sensitivity for clinical use. Here, we combined protein-specific immunoaffinity purification, glycan release, and MS analysis to examine haptoglobin glycosylation of gastric cancer patients for glyco-markers. Age- and sex-matched 60 serum samples (30 cancer patients and 30 healthy controls) were used to profile and quantify haptoglobin N-glycans. A T-test based statistical analysis was performed to identify potential glyco-markers for gastric cancer. Interestingly, abundances of several tri- and tetra-antennary fucosylated N-glycans were increased in gastric cancer patients. Additionally, structural analysis via LC/MS/MS indicated that the fucosylated complex type N-glycans were primarily decorated with antenna fucose, which can be categorized as sialyl-Lea or sialyl-Lex type structures. This platform demonstrates quantitative, structure-specific profiling of haptoglobin glycosylation for the purposes of biomarker discovery for gastric cancer.

Graphical abstract: Glycomic profiling of targeted serum haptoglobin for gastric cancer using nano LC/MS and LC/MS/MS

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Publication details

The article was received on 31 Jul 2016, accepted on 27 Sep 2016 and first published on 28 Sep 2016


Article type: Paper
DOI: 10.1039/C6MB00559D
Citation: Mol. BioSyst., 2016,12, 3611-3621
  • Open access: Creative Commons BY license
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    Glycomic profiling of targeted serum haptoglobin for gastric cancer using nano LC/MS and LC/MS/MS

    S. H. Lee, S. Jeong, J. Lee, I. S. Yeo, M. J. Oh, U. Kim, S. Kim, S. H. Kim, S. Park, J. Kim, S. H. Park, J. H. Kim and H. J. An, Mol. BioSyst., 2016, 12, 3611
    DOI: 10.1039/C6MB00559D

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