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Issue 8, 2016
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High-throughput production of a stable isotope-labeled peptide library for targeted proteomics using a wheat germ cell-free synthesis system

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Abstract

Quantitative proteomic approaches using selected reaction monitoring (SRM) are currently limited by the difficulty in the preparation of reference standards. In this study, we demonstrat the high-throughput production of a reference peptide library using a wheat germ cell-free synthesis system to develop a large-scale SRM assay for targeted proteomics.

Graphical abstract: High-throughput production of a stable isotope-labeled peptide library for targeted proteomics using a wheat germ cell-free synthesis system

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Publication details

The article was received on 23 Mar 2016, accepted on 10 May 2016 and first published on 11 May 2016


Article type: Communication
DOI: 10.1039/C6MB00209A
Citation: Mol. BioSyst., 2016,12, 2389-2393
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    High-throughput production of a stable isotope-labeled peptide library for targeted proteomics using a wheat germ cell-free synthesis system

    N. Takemori, A. Takemori, Y. Tanaka, J. Ishizaki, H. Hasegawa, A. Shiraishi and Y. Ohashi, Mol. BioSyst., 2016, 12, 2389
    DOI: 10.1039/C6MB00209A

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