Issue 8, 2016

High-throughput production of a stable isotope-labeled peptide library for targeted proteomics using a wheat germ cell-free synthesis system

Abstract

Quantitative proteomic approaches using selected reaction monitoring (SRM) are currently limited by the difficulty in the preparation of reference standards. In this study, we demonstrat the high-throughput production of a reference peptide library using a wheat germ cell-free synthesis system to develop a large-scale SRM assay for targeted proteomics.

Graphical abstract: High-throughput production of a stable isotope-labeled peptide library for targeted proteomics using a wheat germ cell-free synthesis system

Supplementary files

Article information

Article type
Communication
Submitted
23 Mar 2016
Accepted
10 May 2016
First published
11 May 2016

Mol. BioSyst., 2016,12, 2389-2393

High-throughput production of a stable isotope-labeled peptide library for targeted proteomics using a wheat germ cell-free synthesis system

N. Takemori, A. Takemori, Y. Tanaka, J. Ishizaki, H. Hasegawa, A. Shiraishi and Y. Ohashi, Mol. BioSyst., 2016, 12, 2389 DOI: 10.1039/C6MB00209A

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