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Issue 4, 2016
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Post-translational control of protein function with light using a LOV-intein fusion protein

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Abstract

Methods for the post-translational control of protein function with light hold much value as tools in cell biology. To this end, we report a fusion protein that consists of DnaE split-inteins, flanking the light sensitive LOV2 domain of Avena sativa. The resulting chimera combines the activities of these two unrelated proteins to enable controlled formation of a functional protein via upregulation of intein splicing with blue light in bacterial and human cells.

Graphical abstract: Post-translational control of protein function with light using a LOV-intein fusion protein

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Publication details

The article was received on 04 Jan 2016, accepted on 01 Mar 2016 and first published on 01 Mar 2016


Article type: Paper
DOI: 10.1039/C6MB00007J
Author version available: Download Author version (PDF)
Citation: Mol. BioSyst., 2016,12, 1388-1393
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    Post-translational control of protein function with light using a LOV-intein fusion protein

    D. C. Jones, I. N. Mistry and A. Tavassoli, Mol. BioSyst., 2016, 12, 1388
    DOI: 10.1039/C6MB00007J

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