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Issue 22, 2016
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One-step immunoassay of C-reactive protein using droplet microfluidics

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Abstract

We present a wash-free high-sensitivity immunoassay of C-reactive proteins with droplet microfluidics. Microbeads are encapsulated within droplets for the immunoassay, and the droplets are scanned by a fluorescence detection platform to quantify the amount of proteins captured on the microbeads. The limit of detection determined by our platform is 0.01 μg mL−1, which is ten times more sensitive than conventional high-sensitivity C-reactive protein assays. With the decrease in diffusion distance within droplets, the immunoassay requires only half of the time required for similar conventional approaches. This approach for carrying out immunoassays can potentially be applied to other biomarkers beyond C-reactive proteins.

Graphical abstract: One-step immunoassay of C-reactive protein using droplet microfluidics

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Publication details

The article was received on 09 Sep 2016, accepted on 06 Oct 2016 and first published on 14 Oct 2016


Article type: Paper
DOI: 10.1039/C6LC01121G
Citation: Lab Chip, 2016,16, 4359-4365
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    One-step immunoassay of C-reactive protein using droplet microfluidics

    M. Y. H. Tang and H. C. Shum, Lab Chip, 2016, 16, 4359
    DOI: 10.1039/C6LC01121G

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