Issue 8, 2016

Translating diagnostic assays from the laboratory to the clinic: analytical and clinical metrics for device development and evaluation

Abstract

As lab-on-a-chip health diagnostic technologies mature, there is a push to translate them from the laboratory to the clinic. For these diagnostics to achieve maximum impact on patient care, scientists and engineers developing the tests should understand the analytical and clinical statistical metrics that determine the efficacy of the test. Appreciating and using these metrics will benefit test developers by providing consistent measures to evaluate analytical and clinical test performance, as well as guide the design of tests that will most benefit clinicians and patients. This paper is broken into four sections that discuss metrics related to general stages of development including: (1) laboratory assay development (analytical sensitivity, limit of detection, analytical selectivity, and trueness/precision), (2) pre-clinical development (diagnostic sensitivity, diagnostic specificity, clinical cutoffs, and receiver-operator curves), (3) clinical use (prevalence, predictive values, and likelihood ratios), and (4) case studies from existing clinical data for tests relevant to the lab-on-a-chip community (HIV, group A strep, and chlamydia). Each section contains definitions of recommended statistical measures, as well as examples demonstrating the importance of these metrics at various stages of the development process. Increasing the use of these metrics in lab-on-a-chip research will improve the rigor of diagnostic performance reporting and provide a better understanding of how to design tests that will ultimately meet clinical needs.

Graphical abstract: Translating diagnostic assays from the laboratory to the clinic: analytical and clinical metrics for device development and evaluation

Supplementary files

Article information

Article type
Tutorial Review
Submitted
06 Jan 2016
Accepted
22 Mar 2016
First published
22 Mar 2016

Lab Chip, 2016,16, 1293-1313

Translating diagnostic assays from the laboratory to the clinic: analytical and clinical metrics for device development and evaluation

M. D. Borysiak, M. J. Thompson and J. D. Posner, Lab Chip, 2016, 16, 1293 DOI: 10.1039/C6LC00015K

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