Jump to main content
Jump to site search

Issue 2, 2016
Previous Article Next Article

Microfluidic device for simultaneous analysis of neutrophil extracellular traps and production of reactive oxygen species

Author affiliations

Abstract

Neutrophil extracellular traps (NETs) were first reported in 2004, and since their discovery, there has been an increasing interest in NETs, how they are formed, their role in controlling infections, and their contribution to disease pathogenesis. Despite this rapid expansion of our understanding of NETs, many details remain unclear including the role of reactive oxygen species (ROS) in the formation of NETs. Further, to study NETs, investigators typically require a large number of cells purified via a lengthy purification regimen. Here, we report a microfluidic device used to quantify both ROS and NET production over time in response to various stimulants, including live bacteria. This device enables ROS and NET analysis using a process that purifies primary human neutrophils in less than 10 minutes and requires only a few microliters of whole blood. Using this device we demonstrate the ability to identify distinct capabilities of neutrophil subsets (including ROS production and NET formation), the ability to use different stimulants/inhibitors, and the ability to effectively use samples stored for up to 8 hours. This device permits the study of ROS and NETs in a user-friendly format and has potential for widespread applications in the study of human disease.

Graphical abstract: Microfluidic device for simultaneous analysis of neutrophil extracellular traps and production of reactive oxygen species

Back to tab navigation

Supplementary files

Publication details

The article was received on 05 Sep 2015, accepted on 24 Dec 2015 and first published on 25 Jan 2016


Article type: Technical Innovation
DOI: 10.1039/C5IB00225G
Citation: Integr. Biol., 2016,8, 243-252
  •   Request permissions

    Microfluidic device for simultaneous analysis of neutrophil extracellular traps and production of reactive oxygen species

    S. F. Moussavi-Harami, K. M. Mladinich, E. K. Sackmann, M. A. Shelef, T. W. Starnes, D. J. Guckenberger, A. Huttenlocher and D. J. Beebe, Integr. Biol., 2016, 8, 243
    DOI: 10.1039/C5IB00225G

Search articles by author

Spotlight

Advertisements