Jump to main content
Jump to site search

Volume 193, 2016
Previous Article Next Article

Single molecular catalysis of a redox enzyme on nanoelectrodes

Author affiliations

Abstract

Due to a high turnover coefficient, redox enzymes can serve as current amplifiers which make it possible to explore their catalytic mechanism by electrochemistry at the level of single molecules. On modified nanoelectrodes, the voltammetric behavior of a horseradish peroxidase (HRP) catalyzed hydroperoxide reduction no longer presents a continuous current response, but a staircase current response. Furthermore, single catalytic incidents were captured through a collision mode at a constant potential, from which the turnover number of HRP can be figured out statistically. In addition, the catalytic behavior is dynamic which may be caused by the orientation status of HRP on the surface of the electrode. This modified nanoelectrode methodology provides an electrochemical approach to investigate the single-molecule catalysis of redox enzymes.

Back to tab navigation

Publication details

The article was received on 30 Mar 2016, accepted on 28 Apr 2016 and first published on 28 Apr 2016


Article type: Paper
DOI: 10.1039/C6FD00061D
Citation: Faraday Discuss., 2016,193, 133-139
  •   Request permissions

    Single molecular catalysis of a redox enzyme on nanoelectrodes

    L. Han, W. Wang, J. Nsabimana, J. Yan, B. Ren and D. Zhan, Faraday Discuss., 2016, 193, 133
    DOI: 10.1039/C6FD00061D

Search articles by author

Spotlight

Advertisements