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Issue 42, 2016
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Fluorescent zinc(II) complexes for gene delivery and simultaneous monitoring of protein expression

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Abstract

Two new zinc(II) complexes, [Zn(L-His)(NIP)]+(1) and [Zn(acac)2(NIP)](2) (where NIP is 2-(naphthalen-1-yl)-1H-imidazo[4,5-f][1,10]phenanthroline, acac = acetyl acetone), have been synthesized and characterized by elemental analysis, UV-vis, fluorescence, IR, 1H NMR and electron spray ionization mass spectroscopies. Gel retardation assay, atomic force microscopy and dynamic light scattering studies show that 1 and 2 can induce the condensation of circular plasmid pBR322 DNA into nanometer size particles under ambient conditions. Treatment of 2 with 5 mM EDTA restored 30% of the supercoiled form of DNA, revealing partial reversibility of DNA condensation. The in vitro transfection experiment demonstrates that the complexes can be used to deliver pCMV-tdTomato-N1 plasmid which expresses red fluorescent protein. The confocal studies show that the fluorescent nature of complexes is advantageous for visualizing the intracellular delivery of metal complexes as well as transfection efficiency using two distinct emission windows.

Graphical abstract: Fluorescent zinc(ii) complexes for gene delivery and simultaneous monitoring of protein expression

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Publication details

The article was received on 20 Jul 2016, accepted on 15 Sep 2016 and first published on 15 Sep 2016


Article type: Paper
DOI: 10.1039/C6DT02871C
Citation: Dalton Trans., 2016,45, 16984-16996
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    Fluorescent zinc(II) complexes for gene delivery and simultaneous monitoring of protein expression

    L. B. Rane, A. N. Kate, S. N. Ramteke, B. V. Shravage, P. P. Kulkarni and A. A. Kumbhar, Dalton Trans., 2016, 45, 16984
    DOI: 10.1039/C6DT02871C

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