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Issue 47, 2016
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Triplex-forming PNA modified with unnatural nucleobases: the role of protonation entropy in RNA binding

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Abstract

Peptide nucleic acid (PNA) modified with unnatural nucleobases enables the formation of a highly stable triplex with a double-stranded RNA at physiological pH. In this communication, we evaluated kinetics and thermodynamics of PNA/RNA triplex formation as a function of both pH and temperature. Protonation entropy was found to be the major factor responsible for the destabilization of the triplex and for the progressive decrease in the association rate at more basic pHs.

Graphical abstract: Triplex-forming PNA modified with unnatural nucleobases: the role of protonation entropy in RNA binding

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Publication details

The article was received on 19 Jul 2016, accepted on 09 Nov 2016 and first published on 09 Nov 2016


Article type: Communication
DOI: 10.1039/C6CP05013A
Citation: Phys. Chem. Chem. Phys., 2016,18, 32002-32006
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    Triplex-forming PNA modified with unnatural nucleobases: the role of protonation entropy in RNA binding

    T. Endoh, C. Annoni, D. Hnedzko, E. Rozners and N. Sugimoto, Phys. Chem. Chem. Phys., 2016, 18, 32002
    DOI: 10.1039/C6CP05013A

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