Jump to main content
Jump to site search

Issue 87, 2016
Previous Article Next Article

A light-up imaging protocol for neutral pH-enhanced fluorescence detection of lysosomal neuraminidase activity in living cells

Author affiliations

Abstract

A lysosome-accessing nanoprobe is designed for recognition of lysosomal neuraminidases (Lyso-Neus), which can cleave the 4-methylumbelliferone moieties of the substrate from the nanoprobe, and lead to the escape of the moieties from acidic lysosomes into the neutral cytosol assisted by cationic poly(ethyleneimine) to light up the pH-responsive fluorescence for visual detection and dynamic tracking of Lyso-Neu activity in living cells.

Graphical abstract: A light-up imaging protocol for neutral pH-enhanced fluorescence detection of lysosomal neuraminidase activity in living cells

Back to tab navigation

Supplementary files

Publication details

The article was received on 17 Sep 2016, accepted on 06 Oct 2016 and first published on 06 Oct 2016


Article type: Communication
DOI: 10.1039/C6CC07574F
Citation: Chem. Commun., 2016,52, 12897-12900
  •   Request permissions

    A light-up imaging protocol for neutral pH-enhanced fluorescence detection of lysosomal neuraminidase activity in living cells

    L. Bao, L. Ding, J. Hui and H. Ju, Chem. Commun., 2016, 52, 12897
    DOI: 10.1039/C6CC07574F

Search articles by author

Spotlight

Advertisements