Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance upgrade on Thursday 4th of May 2017 from 8.00am to 9.00am (BST).

During this time our websites will be offline temporarily. If you have any questions please use the feedback button on this page. We apologise for any inconvenience this might cause and thank you for your patience.

Issue 88, 2016
Previous Article Next Article

Site specific protein O-glucosylation with bacterial toxins

Author affiliations


Using a MALDI-MS based assay, the kinetic parameters for peptide glucosylation using the C. difficile toxin B glycosyltransferase domain were determined. The minimum consensus sequence for glucosylation was YXXTXFXXY and the optimal peptide found was YAPTVFDAY. Using this sequence, homogenous glucosylated proteins could be readily produced.

Graphical abstract: Site specific protein O-glucosylation with bacterial toxins

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 27 Jul 2016, accepted on 07 Oct 2016 and first published on 17 Oct 2016

Article type: Communication
DOI: 10.1039/C6CC06223G
Citation: Chem. Commun., 2016,52, 13024-13026
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    Site specific protein O-glucosylation with bacterial toxins

    Y. Sun, L. M. Willis, H. R. Batchelder and M. Nitz, Chem. Commun., 2016, 52, 13024
    DOI: 10.1039/C6CC06223G

Search articles by author