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Issue 88, 2016
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Site specific protein O-glucosylation with bacterial toxins

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Abstract

Using a MALDI-MS based assay, the kinetic parameters for peptide glucosylation using the C. difficile toxin B glycosyltransferase domain were determined. The minimum consensus sequence for glucosylation was YXXTXFXXY and the optimal peptide found was YAPTVFDAY. Using this sequence, homogenous glucosylated proteins could be readily produced.

Graphical abstract: Site specific protein O-glucosylation with bacterial toxins

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Publication details

The article was received on 27 Jul 2016, accepted on 07 Oct 2016 and first published on 17 Oct 2016


Article type: Communication
DOI: 10.1039/C6CC06223G
Citation: Chem. Commun., 2016,52, 13024-13026
  • Open access: Creative Commons BY-NC license
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    Site specific protein O-glucosylation with bacterial toxins

    Y. Sun, L. M. Willis, H. R. Batchelder and M. Nitz, Chem. Commun., 2016, 52, 13024
    DOI: 10.1039/C6CC06223G

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