Jump to main content
Jump to site search

Issue 87, 2016
Previous Article Next Article

Enzymatic synthesis and reverse transcription of RNAs incorporating 2′-O-carbamoyl uridine triphosphate

Author affiliations

Abstract

Enzymatic synthesis and the reverse transcription of RNAs containing 2′-O-carbamoyl uridine were evaluated. A mild acidic deprotection procedure allowed the synthesis of 2′-O-carbamoyl uridine triphosphate (UcmTP). UcmTP was incorporated correctly into long RNAs, and its fidelity during reverse transcription using SuperScript III was sufficient for RNA aptamer selection.

Graphical abstract: Enzymatic synthesis and reverse transcription of RNAs incorporating 2′-O-carbamoyl uridine triphosphate

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 14 Jul 2016, accepted on 29 Sep 2016 and first published on 29 Sep 2016


Article type: Communication
DOI: 10.1039/C6CC05796A
Citation: Chem. Commun., 2016,52, 12889-12892
  • Open access: Creative Commons BY license
  •   Request permissions

    Enzymatic synthesis and reverse transcription of RNAs incorporating 2′-O-carbamoyl uridine triphosphate

    Y. Masaki, H. Ito, Y. Oda, K. Yamazaki, N. Tago, K. Ohno, N. Ishii, H. Tsunoda, T. Kanamori, A. Ohkubo, M. Sekine and K. Seio, Chem. Commun., 2016, 52, 12889
    DOI: 10.1039/C6CC05796A

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author