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Issue 65, 2016
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Direct detection of nitrotyrosine-containing proteins using an aniline-based oxidative coupling strategy

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Abstract

A convenient two-step method is described for the detection of nitrotyrosine-containing proteins. First, nitrotyrosines are reduced to aminophenols using sodium dithionite. Following this, an oxidative coupling reaction is used to attach anilines bearing fluorescence reporters or affinity probes. Features of this approach include fast reaction times, pmol-level sensitivity, and excellent chemoselectivity.

Graphical abstract: Direct detection of nitrotyrosine-containing proteins using an aniline-based oxidative coupling strategy

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Publication details

The article was received on 01 Jun 2016, accepted on 14 Jul 2016 and first published on 21 Jul 2016


Article type: Communication
DOI: 10.1039/C6CC04575H
Citation: Chem. Commun., 2016,52, 10036-10039
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    Direct detection of nitrotyrosine-containing proteins using an aniline-based oxidative coupling strategy

    R. Sangsuwan, A. C. Obermeyer, P. Tachachartvanich, K. K. Palaniappan and M. B. Francis, Chem. Commun., 2016, 52, 10036
    DOI: 10.1039/C6CC04575H

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