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Issue 28, 2016
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In vitro and in cell analysis of chemically synthesized histone H2A with multiple modifications

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Abstract

The chemical synthetic route to histone H2A is described. An H2A–H2B dimer, histone octamer, and nucleosome were reconstituted with the synthetic H2A. Fluorescein-labeled H2A and multiply modified H2A, which has three different posttranslational modifications, were also synthesized, and applied to live-cell imaging and in vitro nucleosome stability assays, respectively.

Graphical abstract: In vitro and in cell analysis of chemically synthesized histone H2A with multiple modifications

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Publication details

The article was received on 25 Dec 2015, accepted on 04 Mar 2016 and first published on 09 Mar 2016


Article type: Communication
DOI: 10.1039/C5CC10555B
Citation: Chem. Commun., 2016,52, 4999-5002
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    In vitro and in cell analysis of chemically synthesized histone H2A with multiple modifications

    G. Hayashi, T. Sueoka and A. Okamoto, Chem. Commun., 2016, 52, 4999
    DOI: 10.1039/C5CC10555B

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