Issue 28, 2016
From the journal:

Chemical Communications

In vitro and in cell analysis of chemically synthesized histone H2A with multiple modifications†

Gosuke Hayashi,a   Takuma Sueokaa  and  Akimitsu Okamoto*ab  

* Corresponding authors

a Department of Chemistry and Biotechnology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
E-mail: okamoto@chembio.t.u-tokyo.ac.jp

b Address Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan

Abstract

The chemical synthetic route to histone H2A is described. An H2A–H2B dimer, histone octamer, and nucleosome were reconstituted with the synthetic H2A. Fluorescein-labeled H2A and multiply modified H2A, which has three different posttranslational modifications, were also synthesized, and applied to live-cell imaging and in vitro nucleosome stability assays, respectively.

Graphical abstract: In vitro and in cell analysis of chemically synthesized histone H2A with multiple modifications

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Article information

DOI
https://doi.org/10.1039/C5CC10555B
Article type
Communication
Submitted
25 Dec 2015
Accepted
04 Mar 2016
First published
09 Mar 2016

Chem. Commun., 2016,52, 4999-5002

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In vitro and in cell analysis of chemically synthesized histone H2A with multiple modifications

G. Hayashi, T. Sueoka and A. Okamoto, Chem. Commun., 2016, 52, 4999 DOI: 10.1039/C5CC10555B

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