We describe organelle thermometry using an endoplasmic reticulum-targeting small molecule dye and cytosolic mCherry, whose fluorescence lifetimes reduce with increasing temperature and can be monitored by fluorescence lifetime imaging microscopy. The results show that heat production in single myotubes is highly localized and is coupled to a Ca2+ burst.
H. Itoh, S. Arai, T. Sudhaharan, S. Lee, Y. Chang, S. Ishiwata, M. Suzuki and E. B. Lane, Chem. Commun., 2016, 52, 4458 DOI: 10.1039/C5CC09943A
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